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arf gtpases  (Addgene inc)


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    Structured Review

    Addgene inc arf gtpases
    CpoS interacts with Rab <t>GTPases</t> and other C.t . Incs. HeLa cells <t>were</t> <t>transfected</t> with pcDNA 3.1 eGFP-Rab14 or pcMV eGFP Rab 35 and co-infected at an MOI of 5 with C.t. expressing CpoS-HA and either C.t. expressing IPAM-Flag or InaC-Flag. After 24 hpi, CpoS was immunoprecipitated using HA beads, and the elutions were resolved on SDS-PAGE, followed by Western blotting with HA, GFP, and Flag antibodies. Data are representative of three independent experiments.
    Arf Gtpases, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arf gtpases/product/Addgene inc
    Average 93 stars, based on 15 article reviews
    arf gtpases - by Bioz Stars, 2026-06
    93/100 stars

    Images

    1) Product Images from "CpoS-Inc interactions facilitate host cell modulation during Chlamydia trachomatis infection"

    Article Title: CpoS-Inc interactions facilitate host cell modulation during Chlamydia trachomatis infection

    Journal: Infection and Immunity

    doi: 10.1128/iai.00548-25

    CpoS interacts with Rab GTPases and other C.t . Incs. HeLa cells were transfected with pcDNA 3.1 eGFP-Rab14 or pcMV eGFP Rab 35 and co-infected at an MOI of 5 with C.t. expressing CpoS-HA and either C.t. expressing IPAM-Flag or InaC-Flag. After 24 hpi, CpoS was immunoprecipitated using HA beads, and the elutions were resolved on SDS-PAGE, followed by Western blotting with HA, GFP, and Flag antibodies. Data are representative of three independent experiments.
    Figure Legend Snippet: CpoS interacts with Rab GTPases and other C.t . Incs. HeLa cells were transfected with pcDNA 3.1 eGFP-Rab14 or pcMV eGFP Rab 35 and co-infected at an MOI of 5 with C.t. expressing CpoS-HA and either C.t. expressing IPAM-Flag or InaC-Flag. After 24 hpi, CpoS was immunoprecipitated using HA beads, and the elutions were resolved on SDS-PAGE, followed by Western blotting with HA, GFP, and Flag antibodies. Data are representative of three independent experiments.

    Techniques Used: Transfection, Infection, Expressing, Immunoprecipitation, SDS Page, Western Blot



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    Image Search Results


    CpoS interacts with Rab GTPases and other C.t . Incs. HeLa cells were transfected with pcDNA 3.1 eGFP-Rab14 or pcMV eGFP Rab 35 and co-infected at an MOI of 5 with C.t. expressing CpoS-HA and either C.t. expressing IPAM-Flag or InaC-Flag. After 24 hpi, CpoS was immunoprecipitated using HA beads, and the elutions were resolved on SDS-PAGE, followed by Western blotting with HA, GFP, and Flag antibodies. Data are representative of three independent experiments.

    Journal: Infection and Immunity

    Article Title: CpoS-Inc interactions facilitate host cell modulation during Chlamydia trachomatis infection

    doi: 10.1128/iai.00548-25

    Figure Lengend Snippet: CpoS interacts with Rab GTPases and other C.t . Incs. HeLa cells were transfected with pcDNA 3.1 eGFP-Rab14 or pcMV eGFP Rab 35 and co-infected at an MOI of 5 with C.t. expressing CpoS-HA and either C.t. expressing IPAM-Flag or InaC-Flag. After 24 hpi, CpoS was immunoprecipitated using HA beads, and the elutions were resolved on SDS-PAGE, followed by Western blotting with HA, GFP, and Flag antibodies. Data are representative of three independent experiments.

    Article Snippet: HeLa cells, seeded on coverslips, were transfected with GFP-tagged Rab (mentioned above) and Arf GTPases (Addgene #39554, #39556) using Lipofectamine LTX.

    Techniques: Transfection, Infection, Expressing, Immunoprecipitation, SDS Page, Western Blot